Journal: Frontiers in Cell and Developmental Biology

Article Title: Fisetin Promotes Hair Growth by Augmenting TERT Expression

doi: 10.3389/fcell.2020.566617

Figure Lengend Snippet: Screening for polyphenols that activate hTERT transcription in HaCaT cells. (A) Screening for polyphenols that activate hTERT transcription. Polyphenols (10 μM) were added to the HaCaT(hTERTp-EGFP) cells and cultured for 48 h; then, changes in EGFP fluorescence were monitored using an IN Cell Analyzer 1000. (B) The effect of polyphenols on the expression of endogenous hTERT in HaCaT cells was evaluated by qRT-PCR. Statistical significance was determined using a two-sided Student’s t -test. Statistical significance was defined as P < 0.05 (** P < 0.01; *** P < 0.001).

Article Snippet: The HaCaT human keratinocyte cell line (Riken Bioresource Center, Tsukuba, Japan) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Nissui, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; Life Technologies, Gaithersburg, MD, United States) at 37°C in a 5% CO 2 atmosphere.

Techniques: Cell Culture, Fluorescence, Expressing, Quantitative RT-PCR

Journal: Frontiers in Cell and Developmental Biology

Article Title: Fisetin Promotes Hair Growth by Augmenting TERT Expression

doi: 10.3389/fcell.2020.566617

Figure Lengend Snippet: Effects of polyphenols on the expression of cytokine-encoding genes in HaCaT cells. After HaCaT cells were treated with polyphenols, the gene expression levels of IGF-1 (A) , KGF (B) , and TGF- β 1 (C) were measured by qRT-PCR. Statistical significance was determined using a two-sided Student’s t -test. Statistical significance was defined as P < 0.05 (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The HaCaT human keratinocyte cell line (Riken Bioresource Center, Tsukuba, Japan) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Nissui, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; Life Technologies, Gaithersburg, MD, United States) at 37°C in a 5% CO 2 atmosphere.

Techniques: Expressing, Gene Expression, Quantitative RT-PCR

Journal: Frontiers in Cell and Developmental Biology

Article Title: Fisetin Promotes Hair Growth by Augmenting TERT Expression

doi: 10.3389/fcell.2020.566617

Figure Lengend Snippet: Effects of polyphenols on β-catenin activity. The effect of these polyphenols on the activity of β-catenin was evaluated. (A) The TOP-Flash reporter assay was performed to evaluate β-catenin activity in HaCaT cells treated with polyphenols. (B) The effect of polyphenols on AXIN2 expression was evaluated by qRT-PCR. (C,D) The effect of polyphenols on protein expression of β-catenin was evaluated by western blotting using anti-β-catenin antibody. Band intensities were quantitatively determined using ImageJ software. (E) The effect of polyphenols on activity of β-catenin was evaluated by immunofluorescence study using anti-active β-catenin antibody. Statistical significance was determined using a two-sided Student’s t -test. Statistical significance was defined as P < 0.05 (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The HaCaT human keratinocyte cell line (Riken Bioresource Center, Tsukuba, Japan) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Nissui, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; Life Technologies, Gaithersburg, MD, United States) at 37°C in a 5% CO 2 atmosphere.

Techniques: Activity Assay, Reporter Assay, Expressing, Quantitative RT-PCR, Western Blot, Software, Immunofluorescence

Journal: Frontiers in Cell and Developmental Biology

Article Title: Fisetin Promotes Hair Growth by Augmenting TERT Expression

doi: 10.3389/fcell.2020.566617

Figure Lengend Snippet: Effect of polyphenols on the growth of HaCaT cells. (A) After HaCaT cells were treated with 10 μM of polyphenols, cell proliferation was monitored using the Cell Counting Kit-8. (B) The proliferation index on the 3rd day was determined. Statistical significance was determined using a two-sided Student’s t -test. Statistical significance was defined as P < 0.05 (* P < 0.05).

Article Snippet: The HaCaT human keratinocyte cell line (Riken Bioresource Center, Tsukuba, Japan) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Nissui, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; Life Technologies, Gaithersburg, MD, United States) at 37°C in a 5% CO 2 atmosphere.

Techniques: Cell Counting

Journal: Frontiers in Cell and Developmental Biology

Article Title: Fisetin Promotes Hair Growth by Augmenting TERT Expression

doi: 10.3389/fcell.2020.566617

Figure Lengend Snippet: Role of hTERT in the polyphenol-induced effects on HaCaT cells. (A) Relative expression levels of hTERT in HaCaT cells transduced with retroviruses expressing shRNA targeting hTERT (sh-hTERT1 and 2) and scramble shRNA (SCR) were evaluated by qRT-PCR. HaCaT cells whose hTERT expression was reduced by shRNA (sh-hTERT-1 and 2) were treated with polyphenols, and relative β-catenin activity (B) , cytokine gene expression (C,D) , and cell proliferation (E) was determined. Statistical significance was determined using a two-sided Student’s t -test. Statistical significance was defined as P < 0.05 (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The HaCaT human keratinocyte cell line (Riken Bioresource Center, Tsukuba, Japan) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Nissui, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS; Life Technologies, Gaithersburg, MD, United States) at 37°C in a 5% CO 2 atmosphere.

Techniques: Expressing, Transduction, shRNA, Quantitative RT-PCR, Activity Assay, Gene Expression

Journal: International Journal of Medical Sciences

Article Title: Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation

doi: 10.7150/ijms.36484

Figure Lengend Snippet: Gentisic acid induced skin cells proliferation. HaCaT (A) and CCD-986sk (B) cells were treated with various concentrations of gentisic acid. The cell proliferation rate was evaluated with an MTT assay. (** : P < 0.01, *** : P < 0.001)

Article Snippet: The human keratinocyte cell line HaCaT (HaCaT cell was kindly provided by COSMAX BIO, Jecheon, Korea) and human skin fibroblast cell line CCD-986sk (CCD-986sk was purchased from the Korean cell line, Seoul, Korea) was cultured in Dulbecco's Modified Eagle's Medium containing 10% Fetal Bovine Serum and 1% penicillin-streptomycin at 37°C in a 5% CO 2 atmosphere .

Techniques: MTT Assay

Journal: International Journal of Medical Sciences

Article Title: Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation

doi: 10.7150/ijms.36484

Figure Lengend Snippet: Gentisic acid increased in vitro wound healing. HaCaT cells were cultured in a 6-well plate, scratched, and treated with different concentrations of DMSO only (negative control), gentisic acid, or allantoin (positive control). The results were imaged to show scratched wound healing with various compound concentrations.

Article Snippet: The human keratinocyte cell line HaCaT (HaCaT cell was kindly provided by COSMAX BIO, Jecheon, Korea) and human skin fibroblast cell line CCD-986sk (CCD-986sk was purchased from the Korean cell line, Seoul, Korea) was cultured in Dulbecco's Modified Eagle's Medium containing 10% Fetal Bovine Serum and 1% penicillin-streptomycin at 37°C in a 5% CO 2 atmosphere .

Techniques: In Vitro, Cell Culture, Negative Control, Positive Control

Journal: International Journal of Medical Sciences

Article Title: Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation

doi: 10.7150/ijms.36484

Figure Lengend Snippet: Gentisic acid induced ERK1/2 phosphorylation in HaCaT cells. HaCaT cells were treated with various concentration of gentisic acid and the protein extracts were used in Western blot analysis. (A) GAPDH was used as a control. p38, ERK1/2, and JNK, which in their phosphorylated forms are associated with cell proliferation, were detected. (B) p-ERK1/2 was quantified with densitometric analysis and normalized to GAPDH. (* : P < 0.05)

Article Snippet: The human keratinocyte cell line HaCaT (HaCaT cell was kindly provided by COSMAX BIO, Jecheon, Korea) and human skin fibroblast cell line CCD-986sk (CCD-986sk was purchased from the Korean cell line, Seoul, Korea) was cultured in Dulbecco's Modified Eagle's Medium containing 10% Fetal Bovine Serum and 1% penicillin-streptomycin at 37°C in a 5% CO 2 atmosphere .

Techniques: Phospho-proteomics, Concentration Assay, Western Blot, Control

Journal: Molecules

Article Title: Synergetic Effects of Aloe Vera Extract with Trimethylglycine for Targeted Aquaporin 3 Regulation and Long-Term Skin Hydration

doi: 10.3390/molecules29071540

Figure Lengend Snippet: Skin hydration after a single application of the formulas with and without the novel combination.

Article Snippet: The human keratinocyte cell line HaCaT was purchased from MatTek (MatTek Europe, Bratislava, Slovakia).

Techniques: Formulation, Negative Control

Journal: Molecules

Article Title: Synergetic Effects of Aloe Vera Extract with Trimethylglycine for Targeted Aquaporin 3 Regulation and Long-Term Skin Hydration

doi: 10.3390/molecules29071540

Figure Lengend Snippet: Skin hydration after long-term use of the formulas with and without the novel combination.

Article Snippet: The human keratinocyte cell line HaCaT was purchased from MatTek (MatTek Europe, Bratislava, Slovakia).

Techniques: Formulation, Negative Control